| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154284 |
| Chromosome: | chromosome_11 |
| Location: | 2546120 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATACCGTACGGTGCACACAGGTATACACAT |
| Internal bar code: | CCAAATGAAGACATCGCTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1170 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGGACTTTGAGAGCCTG |
| Suggested primer 2: | CGGAGTGATAGTGCTCGTCA |