Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.154296 |
Chromosome: | chromosome 12 |
Location: | 88432 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g483900 | (1 of 3) 3.6.3.2 - Magnesium-importing ATPase / Mg(2+)-importing ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCCCGATCCCCTCAACCCCCCTTCT |
Internal bar code: | GCATAAGACGCGCTTGAGTCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 56 |
LEAP-Seq percent confirming: | 82.6087 |
LEAP-Seq n confirming: | 38 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGATGTCCATCAGCACCT |
Suggested primer 2: | AGGACTGTCTGGTTCCATCG |