Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.154299 |
Chromosome: | chromosome 17 |
Location: | 7081237 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g747147 | (1 of 14) PF10294 - Lysine methyltransferase (Methyltransf_16) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTATGCCGTGCCTGTTTGACCTGTCC |
Internal bar code: | GCTACTGGGATCTGACGGTCCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 304 |
LEAP-Seq percent confirming: | 99.7199 |
LEAP-Seq n confirming: | 1424 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGCTGTCCTGTGTTCTGGA |
Suggested primer 2: | CCAGACACAACCCTGTTCCT |