Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.154311 |
Chromosome: | chromosome 11 |
Location: | 1539621 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467752 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACGGTGCCTGATCCCGTGGAGAATCT |
Internal bar code: | TTTCATTAGCTTTCCGATACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 537 |
LEAP-Seq percent confirming: | 99.4341 |
LEAP-Seq n confirming: | 1230 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCCGCACACACATTTTT |
Suggested primer 2: | ACTGAGGCTGTGTGTGTTGC |