| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154314 |
| Chromosome: | chromosome 1 |
| Location: | 2827848 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016900 | (1 of 1) PF14347 - Domain of unknown function (DUF4399) (DUF4399) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCCGTACGACTCGTGCTGCGATGAAG |
| Internal bar code: | AAAGCCTAGAGGGCCCTTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 96.6429 |
| LEAP-Seq n confirming: | 8262 |
| LEAP-Seq n nonconfirming: | 287 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTGAAGCTATTTGTGC |
| Suggested primer 2: | GGGACTGGTGCTGCTAATGT |