| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154315 |
| Chromosome: | chromosome 2 |
| Location: | 5263090 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106400 | PDAT1,LCA1 | Phospholipid diacylglycerol acyltransferase; (1 of 1) 2.3.1.158 - Phospholipid:diacylglycerol acyltransferase / PDAT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAAGATACGGTAGGCGGCGGGGCGGGG |
| Internal bar code: | GGCTACTGCTGCACCCCTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 99.6726 |
| LEAP-Seq n confirming: | 5480 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCACTCGTACGGAGAGAA |
| Suggested primer 2: | CATGCAGCTTCTTTCCCTCT |