Insertion junction: LMJ.RY0402.154320_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.154320
Chromosome:chromosome 11
Location:1074868
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467675 (1 of 6) PF12796//PF13920 - Ankyrin repeats (3 copies) (Ank_2) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCGCTCATGTTCCTGTTTGGCGGCGAGG
Internal bar code:CCCTTGCACCGGTTCGAGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:450
LEAP-Seq percent confirming:7.36337
LEAP-Seq n confirming:384
LEAP-Seq n nonconfirming:4831
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGTTTGTGTGTGGGTGC
Suggested primer 2:CACCCCTACTTAGCACGCTC