| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154331 |
| Chromosome: | chromosome 2 |
| Location: | 2736575 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093800 | NRX3 | Nucleoredoxin 3; (1 of 3) PTHR13871//PTHR13871:SF38 - THIOREDOXIN // PROTEIN C32D5.8, ISOFORM B-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTGCAACATCCTTATGCTTAGTACGC |
| Internal bar code: | GGGCTAAAAGCTCCCTTCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 86.5471 |
| LEAP-Seq n confirming: | 193 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATTCTTGGGTGCAGGAT |
| Suggested primer 2: | AAACACCACCTCCCAGTCAG |