| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154331 |
| Chromosome: | chromosome 2 |
| Location: | 5493733 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108150 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACCAAGAGGGGTCCGTGGAGGGTGGCCA |
| Internal bar code: | CATAGGGCAAGGAATCCGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 41.826 |
| LEAP-Seq n confirming: | 1269 |
| LEAP-Seq n nonconfirming: | 1765 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCTTTGACGCTTGGAC |
| Suggested primer 2: | AAGGTGGAATTCAACGCAAG |