| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154342 |
| Chromosome: | chromosome 2 |
| Location: | 5735012 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110263 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCAAGCAGCCGCTACTGGTCTCAGTGG |
| Internal bar code: | TTACAACTGGGATAGGGTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 67.517 |
| LEAP-Seq n confirming: | 794 |
| LEAP-Seq n nonconfirming: | 382 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAATGCTGTTTTAGGC |
| Suggested primer 2: | TGACGAGCACTATCACTCCG |