| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154363 |
| Chromosome: | chromosome 16 |
| Location: | 4398632 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671250 | (1 of 3) K12041 - solute carrier family 9 (sodium/hydrogen exchanger), member 6/7 (SLC9A6_7, NHE6_7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCAAACTGGCGAAGGTGAGAGGAGGAT |
| Internal bar code: | CTGACGAAATGCCTTGCTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 99.3592 |
| LEAP-Seq n confirming: | 2326 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTGTAAAGCATCACCTG |
| Suggested primer 2: | ATGCAGTCGTGCAGTGAGTC |