Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.154364 |
Chromosome: | chromosome_3 |
Location: | 5432835 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185050 | FAP53 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGCGGATGGCGTCCACCTGCGCCATGAGT |
Internal bar code: | CCGCTGTTTGACTACCCGTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 660 |
LEAP-Seq percent confirming: | 99.703 |
LEAP-Seq n confirming: | 1343 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGTACCCACAGTCGAAGC |
Suggested primer 2: | GGAGCTGCAAGAGTTCAACC |