| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154366 |
| Chromosome: | chromosome 2 |
| Location: | 649558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078000 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAACTTCTTCACCGACTTCCACTCGG |
| Internal bar code: | GCCGAAGTCGTGGGTTCCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.9196 |
| LEAP-Seq n confirming: | 2487 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCACCACATGACACACA |
| Suggested primer 2: | ATAGTCGGCCAGAACACACC |