Insertion junction: LMJ.RY0402.154370_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154370
Chromosome:chromosome 1
Location:5001814
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g034500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGACGCTGGGCGCGCTGGTTGCCAACGT
Internal bar code:GGACGGTCGGTAGGTGGGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:369
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:474
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGAGGTAAACGACTGGT
Suggested primer 2:TATTCGTGAGTCCGCAAGTG