| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154391 |
| Chromosome: | chromosome 8 |
| Location: | 1792299 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366450 | (1 of 2) PTHR23213:SF30 - DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAACTACCACGGTCTTGCTACCGCTCCG |
| Internal bar code: | AGGGTAGAGGATGTCTACTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACATAAGCATGGCGAGCAG |
| Suggested primer 2: | CCAGCGTACTTGTCCAGGTT |