| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154415 |
| Chromosome: | chromosome 12 |
| Location: | 9545446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546027 | (1 of 2) PF13472 - GDSL-like Lipase/Acylhydrolase family (Lipase_GDSL_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACCATCCCCTGATACCAGTACACGTA |
| Internal bar code: | CGGGGTTGACCGTCGTAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.3789 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCTACTGTGATGCCAC |
| Suggested primer 2: | CCTGCACAAATTGGGTTTCT |