| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154431 |
| Chromosome: | chromosome 10 |
| Location: | 1683579 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430100 | ELG22 | Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCCTGCCCGCACGCGCCCTCGTATGCA |
| Internal bar code: | GAAATATAAAAGGTTACGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.695 |
| LEAP-Seq n confirming: | 7846 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTCGCCGATTAGCTGC |
| Suggested primer 2: | TGGTCGGTTGATATTGCTGA |