Insertion junction: LMJ.RY0402.154439_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.154439
Chromosome:chromosome 17
Location:1591113
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707700 NRM1,DMT1,NRAMP1 (1 of 2) PTHR11706:SF33 - NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 1; Manganese/metal transporter, NRAMP homolog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGGCTCGTTCAAAGTGCTGTCGCCACG
Internal bar code:CAAGGTCGATCGGGGATGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:99.5873
LEAP-Seq n confirming:2413
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTCCTTGGTTGCCTACG
Suggested primer 2:GCAGCGTAGATACGCATTGA