| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154448 |
| Chromosome: | chromosome 13 |
| Location: | 3325339 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586600 | (1 of 1) K08360 - cytochrome b-561 (CYB561) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCTCCTGCGTGGCGGCGCGCCCGCATT |
| Internal bar code: | CGATAAACGCGTGGAAAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 72.7808 |
| LEAP-Seq n confirming: | 1976 |
| LEAP-Seq n nonconfirming: | 739 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATACTGCCAACCACGTCC |
| Suggested primer 2: | ACCGGGCTACAGGAAAAGAT |