| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154448 |
| Chromosome: | chromosome 13 |
| Location: | 3325734 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586600 | (1 of 1) K08360 - cytochrome b-561 (CYB561) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCCATAGCATGAATATGCACCGAGTA |
| Internal bar code: | TAGCAAGATATCGGGGCGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 99.776 |
| LEAP-Seq n confirming: | 891 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAGCCACGCTATCTGGG |
| Suggested primer 2: | ACTAAGATTTGGGTGGCGTG |