| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154478 |
| Chromosome: | chromosome 12 |
| Location: | 5955357 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534550 | PHR4 | (1 of 1) PTHR11455:SF2 - BLUE-LIGHT PHOTORECEPTOR PHR2; Putative photolyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGCTTATGTATGATGTATGGCTAGGGT |
| Internal bar code: | GTATAAAAGTTCATGCGTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 99.5278 |
| LEAP-Seq n confirming: | 6745 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATAGGTGCTGATGGGGG |
| Suggested primer 2: | CCCCAGTTGTCACCTTGAGT |