| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154481 |
| Chromosome: | chromosome_4 |
| Location: | 1229155 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g215250 | FAP26 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTGGACACCGCCGCACTGGCCGCCGAGCC |
| Internal bar code: | TGATAGTAGGCAGCTGTCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 99.5195 |
| LEAP-Seq n confirming: | 1657 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCTTGGCTTCACCAACT |
| Suggested primer 2: | GGCAAATAGGGGTAAGGAGC |