| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154491 |
| Chromosome: | chromosome_1 |
| Location: | 3387701 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g021550 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAACCCGTTGCGCCGCCCGCCGGACCGCC |
| Internal bar code: | GTGATGAGATTTTAAGATTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 96.7599 |
| LEAP-Seq n confirming: | 5913 |
| LEAP-Seq n nonconfirming: | 198 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTCGCCTTCACGAACTC |
| Suggested primer 2: | GCGGATTCTCAAGGTGGATA |