| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154518 |
| Chromosome: | chromosome 1 |
| Location: | 4119142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027100 | IMP2 | Mitochondrial inner membrane signal peptidase; (1 of 1) K09648 - mitochondrial inner membrane protease subunit 2 (IMP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGTAGCGTTGGCCTTGAGCTATGAATCA |
| Internal bar code: | GGCGCCCGGCATGTACAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 513 |
| LEAP-Seq percent confirming: | 99.5826 |
| LEAP-Seq n confirming: | 1193 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGGTGTAGCTTGATATG |
| Suggested primer 2: | TCGAGCTGCTTTGGGTAGTT |