| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154542 |
| Chromosome: | chromosome 10 |
| Location: | 3036297 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441250 | TMEM231 | (1 of 1) K19362 - transmembrane protein 231 (TMEM231); Transmembrane protein 231 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAACTCCAGCACGCCATCCTACTCCA |
| Internal bar code: | GTGTCACAAGACCGGATGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 99.4159 |
| LEAP-Seq n confirming: | 4085 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACGTTTGACGGACCTT |
| Suggested primer 2: | GCTAACTACAGGGTGCGGAG |