| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154543 |
| Chromosome: | chromosome 2 |
| Location: | 6827710 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119150 | (1 of 8) PF00169 - PH domain (PH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACCCACACGCTGCTCCGGGGGTTAAGTG |
| Internal bar code: | CAATGGGAGACACACGCTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.0847 |
| LEAP-Seq n confirming: | 433 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGGTTAAGAAGGGAA |
| Suggested primer 2: | GACTGAGGCTGACCGACTTC |