Insertion junction: LMJ.RY0402.154548_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154548
Chromosome:chromosome 3
Location:7670988
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g203900 (1 of 14) IPR013785 - Aldolase-type TIM barrel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCATTGCTGTGTCATCGCGGGAACACCC
Internal bar code:AAGCCTCCTCGGCCACACACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:582
LEAP-Seq percent confirming:93.4292
LEAP-Seq n confirming:910
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATGGGCTGTAGCTTTGC
Suggested primer 2:ACATCCTCCACCTCGTCTTG