| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.154575 |
| Chromosome: | chromosome 4 |
| Location: | 1520680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213751 | (1 of 43) IPR011989 - Armadillo-like helical | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACAAACACCGGCCGCTGCAGCGGATTAT |
| Internal bar code: | AAGATTCAAAGTCGGTTGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.0512 |
| LEAP-Seq n confirming: | 522 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACTGTACAAGGTGCAGA |
| Suggested primer 2: | TCTTCATTGGTTGGGGAGAG |