| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154585 |
| Chromosome: | chromosome 9 |
| Location: | 3945083 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392430 | (1 of 1) 1.1.1.62 - 17-beta-estradiol 17-dehydrogenase / Estrogen 17-oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGGTGTGGGTCCGCTGGGCTGCCGCC |
| Internal bar code: | CAGGGATAGACTAGGGTGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 87.5912 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCCTTCTCAGTGCAGTA |
| Suggested primer 2: | AAAGGTTGTGCAGTTTTGGG |