| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154615 |
| Chromosome: | chromosome 6 |
| Location: | 6785246 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294876 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCATTGCAGGGGTCCATGTTGCGTGCG |
| Internal bar code: | ATTGCGGGCTCACCCCGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 98.9823 |
| LEAP-Seq n confirming: | 3307 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGTATGTGGGGGAAACAC |
| Suggested primer 2: | TCTTGGAGCTGCTGGTAGGT |