Insertion junction: LMJ.RY0402.154618_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154618
Chromosome:chromosome 1
Location:5309811
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g037150 CLV6 (1 of 1) PTHR11689//PTHR11689:SF14 - CHLORIDE CHANNEL // CHLORIDE CHANNEL PROTEIN 2; ion channel/ voltage-gated chloride channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTTCCACCTAACCCCCCACAGGTGCAG
Internal bar code:GGACATCGACTCACGAGAGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:180
LEAP-Seq percent confirming:99.5833
LEAP-Seq n confirming:239
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGACTCAGCTGGATCAGG
Suggested primer 2:GCCCTCCAGGTAGTCTGTGA