| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154635 |
| Chromosome: | chromosome 10 |
| Location: | 987342 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424850 | SPL5,PRP31 | (1 of 1) K12844 - U4/U6 small nuclear ribonucleoprotein PRP31 (PRPF31); Pre-mRNA-splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAAATGATCGTAGTCCGTTCTCCGCGTG |
| Internal bar code: | TGGATCTCTTCTAAGATTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 97.2318 |
| LEAP-Seq n confirming: | 2810 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGTTGACGGAAGGCATC |
| Suggested primer 2: | TCTGCCCCAAGATTCCTATG |