| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154641 |
| Chromosome: | chromosome 9 |
| Location: | 7189654 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412175 | (1 of 2) 3.7.1.8 - 2,6-dioxo-6-phenylhexa-3-enoate hydrolase / HOHPDA hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCTACAGCCGCAACCGCCATGGAGT |
| Internal bar code: | GCGTTTCAGCGATGATGGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 70.4448 |
| LEAP-Seq n confirming: | 491 |
| LEAP-Seq n nonconfirming: | 206 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGCTGCAGCCTCATCA |
| Suggested primer 2: | GGCAAGTAAAACGACACCGT |