| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154672 |
| Chromosome: | chromosome 6 |
| Location: | 4841053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278500 | TMG3 | (1 of 2) K15445 - tRNA (guanine9-N1)-methyltransferase [EC:2.1.1.221] (TRMT10A, TRM10, RG9MTD2); tRNA (guanine-N1)-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAAATATATGATAGTGTGTATTGACTG |
| Internal bar code: | GGAGGTGTGTTCGACTTACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTACTTGGATAAGCG |
| Suggested primer 2: | CCCGAGCAGTACACAGGAAT |