Insertion junction: LMJ.RY0402.154709_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154709
Chromosome:chromosome 2
Location:6339830
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g115250 POC1 Proteome of centriole protein 1; (1 of 1) K16482 - centriolar protein POC1 (POC1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGATGTACGACACGATGCAACAGTACA
Internal bar code:TTCTAGGACGCGAGCTGATCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:816
LEAP-Seq percent confirming:95.6129
LEAP-Seq n confirming:741
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTTGGAGGACTACACGGG
Suggested primer 2:GTCCTTTGCTGCTGGACTTC