Insertion junction: LMJ.RY0402.154716_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.154716
Chromosome:chromosome 9
Location:2552329
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390750 ELG24 (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 24 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGACTCCCAGCCACAAAACGCGACACCC
Internal bar code:ACACGTGACGAGATGAGGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:99.6438
LEAP-Seq n confirming:1119
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCCCAGACCTGTTGTT
Suggested primer 2:CCCTCAGGTGGTGTTTGAGT