Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.154716 |
Chromosome: | chromosome_9 |
Location: | 2552331 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g390750 | ELG24 | Exostosin-like glycosyltransferase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTGGCTGATGCTTGGGGGCGGCGGGGCTG |
Internal bar code: | CTCGCCGCCCCTGGTTTGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 421 |
LEAP-Seq percent confirming: | 99.9293 |
LEAP-Seq n confirming: | 2827 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTCCCCAGACCTGTTGTT |
Suggested primer 2: | CCCTCAGGTGGTGTTTGAGT |