Insertion junction: LMJ.RY0402.154741_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.154741
Chromosome:chromosome 2
Location:7010292
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g147750 FAP209 Flagellar Associated Protein 209 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCCCGAACCGGCCTTGACCCAAATCTGG
Internal bar code:GGCCGCATTTCACAGGCCTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:803
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:530
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATGATAAGACTGCCTCG
Suggested primer 2:GTCCCCGTCAGCACAATACT