| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.154747 |
| Chromosome: | chromosome 11 |
| Location: | 2397674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474850 | PUS17 | (1 of 2) 5.4.99.29 - 23S rRNA pseudouridine(746) synthase / 23S RNA Psi(746) synthase; RNA pseudouridine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGCATGCTCGGCCGCTGGAGAGGGCGG |
| Internal bar code: | GCTTGTCTGCTGTTTGGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTCCCACAGAAACACAC |
| Suggested primer 2: | GCTAGCGCACTTACCCTGTC |