| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154766 |
| Chromosome: | chromosome 5 |
| Location: | 1312145 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244850 | MOG1,NUOB12 | Ran-interacting protein involved in nuclear trafficking | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATTGACGTGCGTTGCGGAACTAACGCG |
| Internal bar code: | GTGCTGGGGTGAGCGCGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 99.6711 |
| LEAP-Seq n confirming: | 606 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAAGTTATTGCGTGCCC |
| Suggested primer 2: | CGGACATCATGTAGCCCTTT |