| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154793 |
| Chromosome: | chromosome 16 |
| Location: | 6063604 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676200 | (1 of 3) PTHR10751:SF2 - GUANYLATE-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGCACACGGTGCGGATGTGCGTCTTT |
| Internal bar code: | CATCGTAGCAGCGTAGCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 66.5138 |
| LEAP-Seq n confirming: | 580 |
| LEAP-Seq n nonconfirming: | 292 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGAGTACTTTCCAAGCC |
| Suggested primer 2: | CGGTAAAATGGTGGGTATCG |