| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154801 |
| Chromosome: | chromosome 16 |
| Location: | 2367134 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659850 | CGL37 | conserved protein related to shikimate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGAAAGCGAGACCGCAATAAGGAGGGC |
| Internal bar code: | GGAGTTGCTTACTGCGGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 99.5781 |
| LEAP-Seq n confirming: | 1888 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTTTTGCTCACCCTAC |
| Suggested primer 2: | TTTGTGGTTGCTATGGGTCA |