| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154814 |
| Chromosome: | chromosome 7 |
| Location: | 2897724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332500 | AXT2 | (1 of 3) PTHR10994:SF69 - PROLINE-RICH PROTEIN 18; Arabinose chain extension enzyme for HRGP 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACATACGTAGGAGCCCTCATCCCTGCG |
| Internal bar code: | GTACGGTCGCTGTACATGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 99.8821 |
| LEAP-Seq n confirming: | 847 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAAAGCAATCCAAGGTGT |
| Suggested primer 2: | GGGCTGTGGATGTAACGAAT |