| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.154818 |
| Chromosome: | chromosome_13 |
| Location: | 234433 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g563700 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAACGCCCGCCCGTGCCGCCGCCAGCAGC |
| Internal bar code: | GATTAACTCCGCCCCTCCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 85.3333 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTGTGCAATCAAGGGAA |
| Suggested primer 2: | CTGCTTGTTTTCACGACTGC |