Insertion junction: LMJ.RY0402.154819_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.154819
Chromosome:chromosome 2
Location:6055011
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g112650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCTGTCGTGCATGGCATGCTCTGGAAG
Internal bar code:CCTAGTCGTGCGCCCGTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:285
LEAP-Seq percent confirming:95.3846
LEAP-Seq n confirming:186
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCCTCAAAGGAACCGCA
Suggested primer 2:TGGAAACTCGCACACATCAT