| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154819 |
| Chromosome: | chromosome 12 |
| Location: | 5371764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529700 | FAP401 | Putative Flagellar Associated Protein 401 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTCAGCCAGAACAGGTAGTGCTAGTTA |
| Internal bar code: | CAGCCCTAAACTTTGAGTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.573 |
| LEAP-Seq n confirming: | 1166 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACAAGCGCACTTGGATAG |
| Suggested primer 2: | TGCAGCCCCTTCATAATTTC |