| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.154894 |
| Chromosome: | chromosome 12 |
| Location: | 5536145 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531283 | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGGCTGCGAAGCTCTGGCGGATGCAGG |
| Internal bar code: | TGTTCGGATCTAAATCAAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTATGGCCCTGCTTAC |
| Suggested primer 2: | TTCAAAACGTGCTCAAGTCG |