Insertion junction: LMJ.RY0402.154911_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.154911
Chromosome:chromosome_6
Location:2166314
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g265950 DHC3 Flagellar inner arm dynein heavy chain, novel minor type dynein antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCTCGCCGCTGTCGCTGTACGACGTGCAC
Internal bar code:TCTACCCTGTGTAAGGTAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:99.2248
LEAP-Seq n confirming:256
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTCCACCAGGAACATCT
Suggested primer 2:GAGCTGTTCACCGACTCCTC