Insertion junction: LMJ.RY0402.154937_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154937
Chromosome:chromosome 17
Location:3120828
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g721400 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCGACCCACATGCAGCGCACGCCGGAG
Internal bar code:CGTACGGCCAACGCTCAGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:553
LEAP-Seq percent confirming:61.643
LEAP-Seq n confirming:1043
LEAP-Seq n nonconfirming:649
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GACGGTGTCAAATGTTCGTG
Suggested primer 2:ACCTGTAGCGGATCAACACC