Insertion junction: LMJ.RY0402.154968_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.154968
Chromosome:chromosome 1
Location:4711087
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g032500 DNJ8,TPR2 Tetratricopeptide-repeat protein 2; (1 of 1) K09523 - DnaJ homolog subfamily C member 3 (DNAJC3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATTCGCTTCTGACGGCGTTCTTTGTGT
Internal bar code:CCAGGCCGGCGGGTGCTATGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:98.8024
LEAP-Seq n confirming:330
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAAACGTTTCCCTTGTT
Suggested primer 2:TAGGTGCGATGACGTCTGAG